LOCUS pDEST-hsp16-2p 6084 bp DNA circular 03-AUG-2009 FEATURES Location/Qualifiers source 1..6084 /dnas_title="pDEST-hsp16-2p" promoter 49..441 /noet="hsp16-2_promoter" intron 479..520 /note="synthetic intron" misc_recomb 536..660 /note="attR1" misc_recomb 544..550 /note="attR1 break" CDS complement(1089..1394) /gene="ccdB" /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD KVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI" CDS complement(1736..2395) /dnas_title="CamR" /gene="cat" /codon_start=1 /transl_table=11 /product="Chloramphenicol acetyltransferase" /EC_number="2.3.1.28" /function="confers resistance to chloramphenicol" /note="aka ChlR" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG A" regulatory complement(2454..2490) /dnas_title="CamR promoter" /regulatory_class="promoter" /note="includes -35, -10, and transcription start site" regulatory complement(2488..2507) /dnas_title="CAP binding site" /regulatory_class="transcriptional_cis_regulatory_region" regulatory complement(2573..2595) /dnas_title="CAP binding site" /regulatory_class="transcriptional_cis_regulatory_region" misc_recomb 2676..2800 /note="attR2" misc_recomb 2786..2792 /note="attR2 break" polyA_signal 2869..3598 /note=" unc-54_3'_cassette" polyA_signal 3061..3066 /note="AATAAA" promoter 3969..4067 /dnas_title="bla promoter" regulatory 3998..4033 /dnas_title="AmpR promoter" /regulatory_class="promoter" /note="includes -35, -10, and transcription start site" CDS 4263..4928 /note="AmpR" /translation="MMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYSPVTEK HLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRWEPEL NEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSALPA GWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGASLI KHW" rep_origin 5076..5697 /note="pUC origin" regulatory 6011..6041 /dnas_title="lac promoter" /regulatory_class="promoter" regulatory 6047..6067 /dnas_title="lac operator" /regulatory_class="transcriptional_cis_regulatory_region" /note="Lac repressor binding site" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCAAGAGCAT 61 TTGAATCAGA ATATGGAGAA CGGAGCATGA GCATTTTCGA AGTTTTTTAG ATGCACTAGA 121 ACAAAGCGTG TTGGCTTCCT CTGAGCCCGC TTTCCTTATA TACCCGCATT CTGCAGCCTT 181 ACAGAATGTT CTAGAAGGTC CTAGATGCAT TCGTTTGAAA ATACTCCCGG TGGGTGCAAA 241 GAGACGCAGA CGGAAAATGT ATCTGGGTCT CTTTATTGTG TACACTACTT TTCCATGTAC 301 CGAATGTGAG TCGCCCTCCT TTTGCAACAA GCAGCTCGAA TGTTCTAGAA AAAGGTGGAA 361 AATAGTATAA ATACCGTTGA AAATAAATAC CGAACAACAT TTGCTCTAAT TGTGAAATTA 421 GAAATCTTCA ACTATAATCA TCTCACTGGA TCCCCGGGAT TGGCCAAAGG ACCCAAAGGT 481 ATGTTTCGAA TGATACTAAC ATAACATAGA ACATTTTCAG GAGGACCCTT GGCTCACAAG 541 TTTGTACAAA AAAGCTGAAC GAGAAACGTA AAATGATATA AATATCAATA TATTAAATTA 601 GATTTTGCAT AAAAAACAGA CTACATAATA CTGTAAAACA CAACATATCC AGTCACTATG 661 AATCAACTAC TTAGATGGTA TTAGTGACCT GTAGTCGACC GACAGCCTTC CAAATGTTCT 721 TCGGGTGATG CTGCCAACTT AGTCGACCGA CAGCCTTCCA AATGTTCTTC TCAAACGGAA 781 TCGTCGTATC CAGCCTACTC GCTATTGTCC TCAATGCCGT ATTAAATCAT AAAAAGAAAT 841 AAGAAAAAGA GGTGCGAGCC TCTTTTTTGT GTGACAAAAT AAAAACATCT ACCTATTCAT 901 ATACGCTAGT GTCATAGTCC TGAAAATCAT CTGCATCAAG AACAATTTCA CAACTCTTAT 961 ACTTTTCTCT TACAAGTCGT TCGGCTTCAT CTGGATTTTC AGCCTCTATA CTTACTAAAC 1021 GTGATAAAGT TTCTGTAATT TCTACTGTAT CGACCTGCAG ACTGGCTGTG TATAAGGGAG 1081 CCTGACATTT ATATTCCCCA GAACATCAGG TTAATGGCGT TTTTGATGTC ATTTTCGCGG 1141 TGGCTGAGAT CAGCCACTTC TTCCCCGATA ACGGAGACCG GCACACTGGC CATATCGGTG 1201 GTCATCATGC GCCAGCTTTC ATCCCCGATA TGCACCACCG GGTAAAGTTC ACGGGAGACT 1261 TTATCTGACA GCAGACGTGC ACTGGCCAGG GGGATCACCA TCCGTCGCCC GGGCGTGTCA 1321 ATAATATCAC TCTGTACATC CACAAACAGA CGATAACGGC TCTCTCTTTT ATAGGTGTAA 1381 ACCTTAAACT GCATTTCACC AGTCCCTGTT CTCGTCAGCA AAAGAGCCGT TCATTTCAAT 1441 AAACCGGGCG ACCTCAGCCA TCCCTTCCTG ATTTTCCGCT TTCCAGCGTT CGGCACGCAG 1501 ACGACGGGCT TCATTCTGCA TGGTTGTGCT TACCAGACCG GAGATATTGA CATCATATAT 1561 GCCTTGAGCA ACTGATAGCT GTCGCTGTCA ACTGTCACTG TAATACGCTG CTTCATAGCA 1621 CACCTCTTTT TGACATACTT CGGGTATACA TATCAGTATA TATTCTTATA CCGCAAAAAT 1681 CAGCGCGCAA ATACGCATAC TGTTATCTGG CTTTTAGTAA GCCGGATCCA CGCGATTACG 1741 CCCCGCCCTG CCACTCATCG CAGTACTGTT GTAATTCATT AAGCATTCTG CCGACATGGA 1801 AGCCATCACA GACGGCATGA TGAACCTGAA TCGCCAGCGG CATCAGCACC TTGTCGCCTT 1861 GCGTATAATA TTTGCCCATG GTGAAAACGG GGGCGAAGAA GTTGTCCATA TTGGCCACGT 1921 TTAAATCAAA ACTGGTGAAA CTCACCCAGG GATTGGCTGA GACGAAAAAC ATATTCTCAA 1981 TAAACCCTTT AGGGAAATAG GCCAGGTTTT CACCGTAACA CGCCACATCT TGCGAATATA 2041 TGTGTAGAAA CTGCCGGAAA TCGTCGTGGT ATTCACTCCA GAGCGATGAA AACGTTTCAG 2101 TTTGCTCATG GAAAACGGTG TAACAAGGGT GAACACTATC CCATATCACC AGCTCACCGT 2161 CTTTCATTGC CATACGGAAT TCCGGATGAG CATTCATCAG GCGGGCAAGA ATGTGAATAA 2221 AGGCCGGATA AAACTTGTGC TTATTTTTCT TTACGGTCTT TAAAAAGGCC GTAATATCCA 2281 GCTGAACGGT CTGGTTATAG GTACATTGAG CAACTGACTG AAATGCCTCA AAATGTTCTT 2341 TACGATGCCA TTGGGATATA TCAACGGTGG TATATCCAGT GATTTTTTTC TCCATTTTAG 2401 CTTCCTTAGC TCCTGAAAAT CTCGATAACT CAAAAAATAC GCCCGGTAGT GATCTTATTT 2461 CATTATGGTG AAAGTTGGAA CCTCTTACGT GCCGATCAAC GTCTCATTTT CGCCAAAAGT 2521 TGGCCCAGGG CTTCCCGGTA TCAACAGGGA CACCAGGATT TATTTATTCT GCGAAGTGAT 2581 CTTCCGTCAC AGGTATTTAT TCGGCGCAAA GTGCGTCGGG TGATGCTGCC AACTTAGTCG 2641 ACTACAGGTC ACTAATACCA TCTAAGTAGT TGATTCATAG TGACTGGATA TGTTGTGTTT 2701 TACAGTATTA TGTAGTCTGT TTTTTATGCA AAATCTAATT TAATATATTG ATATTTATAT 2761 CATTTTACGT TTCTCGTTCA GCTTTCTTGT ACAAAGTGGT CGGCCGCTGT CATCAGAGTA 2821 AGTTTAAACT GAGTTCTACT AACTAACGAG TAATATTTAA ATTTTCAGCA TCTCGCGCCC 2881 GTGCCTCTGA CTTCTAAGTC CAATTACTCT TCAACATCCC TACATGCTCT TTCTCCCTGT 2941 GCTCCCACCC CCTATTTTTG TTATTATCAA AAAACTTCTC TTAATTTCTT TGTTTTTTAG 3001 CTTCTTTTAA GTCACCTCTA ACAATGAAAT TGTGTAGATT CAAAAATAGA ATTAATTCGT 3061 AATAAAAAGT CGAAAAAAAT TGTGCTCCCT CCCCCCATTA ATAATAATTC TATCCCAAAA 3121 TCTACACAAT GTTCTGTGTA CACTTCTTAT GTTTTTTACT TCTGATAAAT TTTTTTGAAA 3181 CATCATAGAA AAAACCGCAC ACAAAATACC TTATCATATG TTACGTTTCA GTTTATGACC 3241 GCAATTTTTA TTTCTTCGCA CGTCTGGGCC TCTCATGACG TCAAATCATG CTCATCGTGA 3301 AAAAGTTTTG GAGTATTTTT GGAATTTTTC AATCAAGTGA AAGTTTATGA AATTAATTTT 3361 CCTGCTTTTG CTTTTTGGGG GTTTCCCCTA TTGTTTGTCA AGAGTTTCGA GGACGGCGTT 3421 TTTCTTGCTA AAATCACAAG TATTGATGAG CACGATGCAA GAAAGATCGG AAGAAGGTTT 3481 GGGTTTGAGG CTCAGTGGAA GGTGAGTAGA AGTTGATAAT TTGAAAGTGG AGTAGTGTCT 3541 ATGGGGTTTT TGCCTTAAAT GACAGAATAC ATTCCCAATA TACCAAACAT AACTGTTTCC 3601 TACTAGTCGG CCGTACGGGC CCTTTCGTCT CGCGCGTTTC GGTGATGACG GTGAAAACCT 3661 CTGACACATG CAGCTCCCGG AGACGGTCAC AGCTTGTCTG TAAGCGGATG CCGGGAGCAG 3721 ACAAGCCCGT CAGGGCGCGT CAGCGGGTGT TGGCGGGTGT CGGGGCTGGC TTAACTATGC 3781 GGCATCAGAG CAGATTGTAC TGAGAGTGCA CCATATGCGG TGTGAAATAC CGCACAGATG 3841 CGTAAGGAGA AAATACCGCA TCAGGCGGCC TTAAGGGCCT CGTGATACGC CTATTTTTAT 3901 AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT CGGGGAAATG 3961 TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT CCGCTCATGA 4021 GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG AGTATTCAAC 4081 ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT TTTGCTCACC 4141 CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA GTGGGTTACA 4201 TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA GAACGTTTTC 4261 CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT ATTGACGCCG 4321 GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT GAGTACTCAC 4381 CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC AGTGCTGCCA 4441 TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA GGACCGAAGG 4501 AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT CGTTGGGAAC 4561 CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT GTAGCAATGG 4621 CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC CGGCAACAAT 4681 TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG GCCCTTCCGG 4741 CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC GGTATCATTG 4801 CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG ACGGGGAGTC 4861 AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA CTGATTAAGC 4921 ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA AAACTTCATT 4981 TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC AAAATCCCTT 5041 AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA GGATCTTCTT 5101 GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA CCGCTACCAG 5161 CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA ACTGGCTTCA 5221 GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC CACCACTTCA 5281 AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA GTGGCTGCTG 5341 CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA CCGGATAAGG 5401 CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG CGAACGACCT 5461 ACACCGAACT GAGATACCTA CAGCGTGAGC ATTGAGAAAG CGCCACGCTT CCCGAAGGGA 5521 GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC ACGAGGGAGC 5581 TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG 5641 AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG 5701 CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT 5761 TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC 5821 GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCCAATAC 5881 GCAAACCGCC TCTCCCCGCG CGTTGGCCGA TTCATTAATG CAGCTGGCAC GACAGGTTTC 5941 CCGACTGGAA AGCGGGCAGT GAGCGCAACG CAATTAATGT GAGTTAGCTC ACTCATTAGG 6001 CACCCCAGGC TTTACACTTT ATGCTTCCGG CTCGTATGTT GTGTGGAATT GTGAGCGGAT 6061 AACAATTTCA CACAGGAAAC AGCT //