LOCUS pDEST-hsp16-41p 6092 bp DNA linear 22-SEP-2010 FEATURES Location/Qualifiers source 1..6092 /dnas_title="pDEST-hsp16-41p" promoter 49..448 /note="hsp16-41_promoter" intron 487..528 /note="synthetic intron" misc_recomb 544..668 /note="attR1" misc_recomb 552..558 /note="attR1 break" CDS complement(1097..1402) /gene="ccdB" /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD KVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI" CDS complement(1744..2403) /dnas_title="CamR" /gene="cat" /codon_start=1 /transl_table=11 /product="Chloramphenicol acetyltransferase" /EC_number="2.3.1.28" /function="confers resistance to chloramphenicol" /note="aka ChlR" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG A" regulatory complement(2462..2498) /dnas_title="CamR promoter" /regulatory_class="promoter" /note="includes -35, -10, and transcription start site" regulatory complement(2496..2515) /dnas_title="CAP binding site" /regulatory_class="transcriptional_cis_regulatory_region" regulatory complement(2581..2603) /dnas_title="CAP binding site" /regulatory_class="transcriptional_cis_regulatory_region" misc_recomb 2684..2808 /note="attR2" misc_recomb 2794..2800 /note="attR2 break" polyA_signal 2877..3606 /note="unc-54_3'_cassette" polyA_signal 3069..3074 /note="AATAAA" promoter 3977..4075 /dnas_title="bla promoter" regulatory 4006..4041 /dnas_title="AmpR promoter" /regulatory_class="promoter" /note="includes -35, -10, and transcription start site" CDS 4076..4936 /note="AmpR" rep_origin 5084..5705 /note="pUC origin" regulatory 6019..6049 /dnas_title="lac promoter" /regulatory_class="promoter" regulatory 6055..6075 /dnas_title="lac operator" /regulatory_class="transcriptional_cis_regulatory_region" /note="Lac repressor binding site" ORIGIN 1 ATGACCATGA TTACGCCAAG CTTGCATGCC TGCAGGTCGA CTCTAGAGGA TCACCAAAAA 61 CGGAACGTTG AGCTGGACGG AAATAGTGGT AAAGTGACAT GATTATAGTT TGAAGATcTC 121 TAATTTCACA ATTAGAGCAA ATGTTGTTCG GTATTTATTT TCAACGGTAT TTATACTATT 181 TTCCACCTTT TTCTAGAACA TTCGAGCTGC TTGTTGCAAA AGGAGGGCGA CTCACATTCG 241 GTACATGGAA AAGTAGTGTA CACAATAAAG AGACCCAGAT ACATTTTCCG TCTGCGTCTC 301 TTTGCACCCA CCGGGAGTAT TTTCAAACGA ATGCATCTAG GACCTTCTAG AACATTCTGT 361 AAGGCTGCAG AATGCGGGTA TATAAGGAAA GCGGGCTCAG AGGAAGCCAA CACGCTTTGT 421 TCTAGTGCAT CTAAAAAACT TCGAAAATCC TCATCGGATC CCCGGGATTG GCCAAAGGAC 481 CCAAAGGTAT GTTTCGAATG ATACTAACAT AACATAGAAC ATTTTCAGGA GGACCCTTGG 541 CTCACAAGTT TGTACAAAAA AGCTGAACGA GAAACGTAAA ATGATATAAA TATCAATATA 601 TTAAATTAGA TTTTGCATAA AAAACAGACT ACATAATACT GTAAAACACA ACATATCCAG 661 TCACTATGAA TCAACTACTT AGATGGTATT AGTGACCTGT AGTCGACCGA CAGCCTTCCA 721 AATGTTCTTC GGGTGATGCT GCCAACTTAG TCGACCGACA GCCTTCCAAA TGTTCTTCTC 781 AAACGGAATC GTCGTATCCA GCCTACTCGC TATTGTCCTC AATGCCGTAT TAAATCATAA 841 AAAGAAATAA GAAAAAGAGG TGCGAGCCTC TTTTTTGTGT GACAAAATAA AAACATCTAC 901 CTATTCATAT ACGCTAGTGT CATAGTCCTG AAAATCATCT GCATCAAGAA CAATTTCACA 961 ACTCTTATAC TTTTCTCTTA CAAGTCGTTC GGCTTCATCT GGATTTTCAG CCTCTATACT 1021 TACTAAACGT GATAAAGTTT CTGTAATTTC TACTGTATCG ACCTGCAGAC TGGCTGTGTA 1081 TAAGGGAGCC TGACATTTAT ATTCCCCAGA ACATCAGGTT AATGGCGTTT TTGATGTCAT 1141 TTTCGCGGTG GCTGAGATCA GCCACTTCTT CCCCGATAAC GGAGACCGGC ACACTGGCCA 1201 TATCGGTGGT CATCATGCGC CAGCTTTCAT CCCCGATATG CACCACCGGG TAAAGTTCAC 1261 GGGAGACTTT ATCTGACAGC AGACGTGCAC TGGCCAGGGG GATCACCATC CGTCGCCCGG 1321 GCGTGTCAAT AATATCACTC TGTACATCCA CAAACAGACG ATAACGGCTC TCTCTTTTAT 1381 AGGTGTAAAC CTTAAACTGC ATTTCACCAG TCCCTGTTCT CGTCAGCAAA AGAGCCGTTC 1441 ATTTCAATAA ACCGGGCGAC CTCAGCCATC CCTTCCTGAT TTTCCGCTTT CCAGCGTTCG 1501 GCACGCAGAC GACGGGCTTC ATTCTGCATG GTTGTGCTTA CCAGACCGGA GATATTGACA 1561 TCATATATGC CTTGAGCAAC TGATAGCTGT CGCTGTCAAC TGTCACTGTA ATACGCTGCT 1621 TCATAGCACA CCTCTTTTTG ACATACTTCG GGTATACATA TCAGTATATA TTCTTATACC 1681 GCAAAAATCA GCGCGCAAAT ACGCATACTG TTATCTGGCT TTTAGTAAGC CGGATCCACG 1741 CGATTACGCC CCGCCCTGCC ACTCATCGCA GTACTGTTGT AATTCATTAA GCATTCTGCC 1801 GACATGGAAG CCATCACAGA CGGCATGATG AACCTGAATC GCCAGCGGCA TCAGCACCTT 1861 GTCGCCTTGC GTATAATATT TGCCCATGGT GAAAACGGGG GCGAAGAAGT TGTCCATATT 1921 GGCCACGTTT AAATCAAAAC TGGTGAAACT CACCCAGGGA TTGGCTGAGA CGAAAAACAT 1981 ATTCTCAATA AACCCTTTAG GGAAATAGGC CAGGTTTTCA CCGTAACACG CCACATCTTG 2041 CGAATATATG TGTAGAAACT GCCGGAAATC GTCGTGGTAT TCACTCCAGA GCGATGAAAA 2101 CGTTTCAGTT TGCTCATGGA AAACGGTGTA ACAAGGGTGA ACACTATCCC ATATCACCAG 2161 CTCACCGTCT TTCATTGCCA TACGGAATTC CGGATGAGCA TTCATCAGGC GGGCAAGAAT 2221 GTGAATAAAG GCCGGATAAA ACTTGTGCTT ATTTTTCTTT ACGGTCTTTA AAAAGGCCGT 2281 AATATCCAGC TGAACGGTCT GGTTATAGGT ACATTGAGCA ACTGACTGAA ATGCCTCAAA 2341 ATGTTCTTTA CGATGCCATT GGGATATATC AACGGTGGTA TATCCAGTGA TTTTTTTCTC 2401 CATTTTAGCT TCCTTAGCTC CTGAAAATCT CGATAACTCA AAAAATACGC CCGGTAGTGA 2461 TCTTATTTCA TTATGGTGAA AGTTGGAACC TCTTACGTGC CGATCAACGT CTCATTTTCG 2521 CCAAAAGTTG GCCCAGGGCT TCCCGGTATC AACAGGGACA CCAGGATTTA TTTATTCTGC 2581 GAAGTGATCT TCCGTCACAG GTATTTATTC GGCGCAAAGT GCGTCGGGTG ATGCTGCCAA 2641 CTTAGTCGAC TACAGGTCAC TAATACCATC TAAGTAGTTG ATTCATAGTG ACTGGATATG 2701 TTGTGTTTTA CAGTATTATG TAGTCTGTTT TTTATGCAAA ATCTAATTTA ATATATTGAT 2761 ATTTATATCA TTTTACGTTT CTCGTTCAGC TTTCTTGTAC AAAGTGGTCG GCCGCTGTCA 2821 TCAGAGTAAG TTTAAACTGA GTTCTACTAA CTAACGAGTA ATATTTAAAT TTTCAGCATC 2881 TCGCGCCCGT GCCTCTGACT TCTAAGTCCA ATTACTCTTC AACATCCCTA CATGCTCTTT 2941 CTCCCTGTGC TCCCACCCCC TATTTTTGTT ATTATCAAAA AACTTCTCTT AATTTCTTTG 3001 TTTTTTAGCT TCTTTTAAGT CACCTCTAAC AATGAAATTG TGTAGATTCA AAAATAGAAT 3061 TAATTCGTAA TAAAAAGTCG AAAAAAATTG TGCTCCCTCC CCCCATTAAT AATAATTCTA 3121 TCCCAAAATC TACACAATGT TCTGTGTACA CTTCTTATGT TTTTTACTTC TGATAAATTT 3181 TTTTGAAACA TCATAGAAAA AACCGCACAC AAAATACCTT ATCATATGTT ACGTTTCAGT 3241 TTATGACCGC AATTTTTATT TCTTCGCACG TCTGGGCCTC TCATGACGTC AAATCATGCT 3301 CATCGTGAAA AAGTTTTGGA GTATTTTTGG AATTTTTCAA TCAAGTGAAA GTTTATGAAA 3361 TTAATTTTCC TGCTTTTGCT TTTTGGGGGT TTCCCCTATT GTTTGTCAAG AGTTTCGAGG 3421 ACGGCGTTTT TCTTGCTAAA ATCACAAGTA TTGATGAGCA CGATGCAAGA AAGATCGGAA 3481 GAAGGTTTGG GTTTGAGGCT CAGTGGAAGG TGAGTAGAAG TTGATAATTT GAAAGTGGAG 3541 TAGTGTCTAT GGGGTTTTTG CCTTAAATGA CAGAATACAT TCCCAATATA CCAAACATAA 3601 CTGTTTCCTA CTAGTCGGCC GTACGGGCCC TTTCGTCTCG CGCGTTTCGG TGATGACGGT 3661 GAAAACCTCT GACACATGCA GCTCCCGGAG ACGGTCACAG CTTGTCTGTA AGCGGATGCC 3721 GGGAGCAGAC AAGCCCGTCA GGGCGCGTCA GCGGGTGTTG GCGGGTGTCG GGGCTGGCTT 3781 AACTATGCGG CATCAGAGCA GATTGTACTG AGAGTGCACC ATATGCGGTG TGAAATACCG 3841 CACAGATGCG TAAGGAGAAA ATACCGCATC AGGCGGCCTT AAGGGCCTCG TGATACGCCT 3901 ATTTTTATAG GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG 3961 GGGAAATGTG CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC 4021 GCTCATGAGA CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG 4081 TATTCAACAT TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT 4141 TGCTCACCCA GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT 4201 GGGTTACATC GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA 4261 ACGTTTTCCA ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTAT 4321 TGACGCCGGG CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA 4381 GTACTCACCA GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG 4441 TGCTGCCATA ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG 4501 ACCGAAGGAG CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG 4561 TTGGGAACCG GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGT 4621 AGCAATGGCA ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG 4681 GCAACAATTA ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC 4741 CCTTCCGGCT GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG 4801 TATCATTGCA GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC 4861 GGGGAGTCAG GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT 4921 GATTAAGCAT TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA 4981 ACTTCATTTT TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA 5041 AATCCCTTAA CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG 5101 ATCTTCTTGA GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC 5161 GCTACCAGCG GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC 5221 TGGCTTCAGC AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA 5281 CCACTTCAAG AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT 5341 GGCTGCTGCC AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC 5401 GGATAAGGCG CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG 5461 AACGACCTAC ACCGAACTGA GATACCTACA GCGTGAGCAT TGAGAAAGCG CCACGCTTCC 5521 CGAAGGGAGA AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC 5581 GAGGGAGCTT CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT 5641 CTGACTTGAG CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC 5701 CAGCAACGCG GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT 5761 TCCTGCGTTA TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC 5821 CGCTCGCCGC AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG 5881 CCCAATACGC AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA GCTGGCACGA 5941 CAGGTTTCCC GACTGGAAAG CGGGCAGTGA GCGCAACGCA ATTAATGTGA GTTAGCTCAC 6001 TCATTAGGCA CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATGTTGT GTGGAATTGT 6061 GAGCGGATAA CAATTTCACA CAGGAAACAG CT //