LOCUS peft-3p-RET-1E4E 8374 bp DNA circular 04-SEP-2013 FEATURES Location/Qualifiers source 1..8374 /dnas_title="peft-3p-RET-1E4E5(+1)E6-(+2)GGS6-EGFP" promoter 23..627 /note="derived_from_eft-3" misc_feature 594..595 /note="SL1 trans-splice acceptor; see cm01d1.5" misc_feature 594..595 /note="SL2 trans-splice acceptor; see yk1281a09.5" mRNA join(595..650,693..770,2435..2516,4281..5361) /dnas_title="RET-1E4/E5(+1)/E6-GGS6-EGFP" mRNA join(595..650,693..770,4281..5361) /dnas_title="RET-1E4/E6-GGS6-EGFP" intron 651..692 /note="synthetic_intron" misc_recomb 708..732 /note="attB1" misc_recomb 716..722 /note="attB1 break" CDS join(738..770,2435..2516,4281..5110) /dnas_title="RET-1E4/E5(+1)/E6-GGS6-EGFP" /translation="MVFGSLGSDDMKPGSQDDGFVFIERNEANEATFKEESEDVFPSQ RRHRKEFNFVYKSWGGSGGSGGSGGSGGSGGSVSKGEELFTGVVPILVELDGDVNGHK FSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFF KSAMPEGYVQERTIFFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLE YNYNSHNVYIMADKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHY LSTQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK" CDS join(738..770,4281..4397) /dnas_title="RET-1E4/E6*" /translation="MVFGSLGSDDMMSSHHNDVIEKNSTLYTKVGEDQEDQEDQEDQE DQEDQ" gene 741..4317 /note="derived from ret-1" variation 2500..2500 /note="+1 insertion" misc_recomb 4318..4338 /note="attB5" /note="CAACTTTGTATACAAAAGTTG" misc_recomb 4325..4331 /note="attB5 break" CDS 4340..4393 /note="(GGS)X6" CDS 4394..5110 /dnas_title="Enhanced Green Fluorescent Protein" /gene="egfp" /codon_start=1 /product="Enhanced Green Fluorescent Protein" /db_xref="PID:g1373322" /transl_table=11 /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKD DGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNG IKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHM VLLEFVTAAGITLGMDELYK" misc_recomb 5112..5136 /note="attB2" /note="ACCCAGCTTTCTTGTACAAAGTGGT" misc_recomb 5122..5128 /note="attB2 break" polyA_signal 5138..5888 /note="unc-54 3'_cassette" polyA_signal 5350..5355 /note="polyA" promoter 6259..6357 /dnas_title="bla promoter" regulatory 6288..6323 /dnas_title="AmpR promoter" /regulatory_class="promoter" /note="includes -35, -10, and transcription start site" /note="includes -35, -10, and transcription start site" CDS 6358..7218 /dnas_title="Ampicillin (bla) resistance gene" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 7366..7987 /note="pUC origin" regulatory 8301..8331 /dnas_title="lac promoter" /regulatory_class="promoter" /note="lac promoter" regulatory 8337..8357 /dnas_title="lac operator" /regulatory_class="transcriptional_cis_regulatory_region" /note="Lac repressor binding site" /note="Lac repressor binding site" ORIGIN 1 ATGACCATGA TTACGCCAAG CTtgtcaact tccattggtt cttccattgt ttctgttaaa 61 ttaatgaatt tttcataaaa taaagacatt atacaatata aaaatgaaga atttattgaa 121 aataaactgc cagagagaaa aagtatgcaa cactcccgcc gagagtgttt gaaatggtgt 181 acggtacatt ttcgtgctag gagttagatg tgcaggcagc aacgagaggg ggagagattt 241 ttttgggcct tgtgaaatta acgtgagttt tctggtcatc tgactaatca tgttggtttt 301 ttgttggttt attttgtttt tatctttgtt tttatccaga ttaggaaatt taaattttat 361 gaatttataa tgaggtcaaa cattcagtcc cagcgttttt cctgttctca ctgtttagtc 421 gaatttttat tttaggcttt caacaaatgt tctaactgtc ttatttgtga cctcactttt 481 tatatttttt taatttttaa aaatattaga agtttctagg ataatttttt cgacttttat 541 tctctctacc gtccgcactc ttcttacttt taaattaaat tgtttttttt tcagttggga 601 aacactttgc tcactccgta gcagccaGGG ATTGGCCAAA GGACCCAAAG gtatgtttcg 661 aatgatacta acataacata gaacattttc agGAGGACCC TTGGCTCACA AGTTTGTACA 721 AAAAAGCAGG CTccaccatg GTATTTGGAA GCCTTGGATC TGACGACATG gttagtttat 781 ttttatttgc aaaattttgg ttacatttta taatttaaac gaggaatgtg gtgaatgaat 841 tgaggaatct ttatggcctg tctctctagg tttgagcttt aaattttctg aaattaataa 901 ttgttaaagt caaatttaag gttcaaaacg tttgtgaatg cgttgaatgc ttgttcagta 961 tatttctatc ctacttttaa attcaatctg aaatcagata ggtcttatta gataggctta 1021 ggctgccaaa aattgtgctc ctgactgagt ttgaactggg aaactgtttt caaaatattt 1081 taaatttatc actttcgagt ccccgtgcaa ttgctccaag ttcaaatttt catttatttt 1141 ttatgatttt agagaaatag agaagtagga aaagatttag gatgcttgtt aggtttgttt 1201 ttcaattttg aaactttttt catcaaactc gatcaaaagt ctaaaatttt aattaggaaa 1261 gggacaactt tgattgattc taaaaaattt gaacaatttc gagtcggttt cttttagttc 1321 aaagatattt tctacagttt ttatagaagt ttggtgaaac tgaaaatctg aaattttata 1381 tttacaatta gtaaaagtct atcaatgact cttacactaa ataaaccctt ccaaaaaaag 1441 tccatataac tgtctggaga tcaaatactt tttttctccc aacttttcac actctcatca 1501 aaaccatttc tcgtggaacc atcaattttt attggcttca ttctcgttcg attcttcctc 1561 ttttccatca ccaaatgcac atacaaactc gcggaaaaaa gaaactaaaa gccaaacaaa 1621 tcgttacttt tctatccttt tccgaggtgg aaaaaagggt gacgctccac cacaatttta 1681 taaaaggaag attggagatt ccgtgaacct gcccattttc gttccttttc tgttcctttt 1741 gggactagtc gtccatgtaa aattataaaa ttgcgctgcg ccgcatacac tcgcatactt 1801 ttgccccttc ttctctctaa ggctttttgg tggtaactgg atagtggaag attctcaaat 1861 ttcttcgtct ttttccatat tttactacta agatataagt gagccacgag ctgctatgat 1921 tgtcaaatca tgacaatgat tttcggtgct tcttctgtaa ttatttctga aaatttggat 1981 gacgaatcag agagacaggt cttggtttat tatcagtttg tcaaaaaata tattgtgttg 2041 aaaataatgt cgccccaata attttgcaag cctgccataa agttaatcgt gtcgattcta 2101 ttaggacttg tgtagtccag gaggagaagt gttcatttat aattttaaat gttttttttg 2161 tatttaaaaa caatgaataa tggattgatc attttgaatg ttgagttcag aagagtttgg 2221 cgaaaaactg aaaattattt taatacatta aaacttttcc tagtaaaata catcgtaaat 2281 tggcatgata aaccgtgtgg cttgcaaaat tcaaggagct ctagcaggac aaaatgggaa 2341 actgatatat attggtgttg caagtgacta tattttcaaa aaacttggat attgtaaaaa 2401 tgcatgaatt tgtgaggtga tgtgtctaat tcagAAGCCA GGATCTCAGG ACGACGGATT 2461 CGTATTCATC GAACGAAACG AGGCAAACGA AGCAACTTTt AAAGAAGAAT CAGAAGgttg 2521 tgaatgagtt actttttctg tttttttttt agttttgttc tatgtctgtc agctccttaa 2581 tttgttgtag aactgtcagt gtttgtcaag tacacccaac cgctcctttt tgctatatca 2641 catgacccca atggaacaac caacaaacga aaaatatatt ttgagaaaaa ccggaaaggt 2701 gatcgcaaaa aaatattttc tcattttatt ttggtttgat agtgcggtaa tcaaagcgag 2761 tgtgaccgaa gaaaatcggt tgattactca ttgctatcga acactttttt actttgcaat 2821 actttatgat gactcattgg gaggcgtttg ctagattttt taacaagaat ttgaattatt 2881 ttcttttaat ccaaaaaaat tttattttat tttttctatt tttgttgatt tttcgataat 2941 tgaattcaga aaaaccaaca aaacgtacga aaaagaaatg aacttcgatg ttttgcaaaa 3001 aatattaaag cgcgcggcga gacccatccg cagaatttaa ccacctccct tgaataccgt 3061 agcatttctc aagaaaacac attttatttt caaatttaat tttttctgaa ttttacaagt 3121 gaatcctcat tgcggcgcca ctattttatt tttaatattt cacaactcaa aatttaaaga 3181 aaaaagtatt gctcacaaat tctcagcaat atttgttaaa actgttcaaa cctgcaaact 3241 ttcgaaacac tctccaaaag tgtcagcaat taatgagtca attttccgcg cgcgccacga 3301 gaaaattgct ttggaactgc ccaaagaccg cccgttctct tgttttccgg tcactcgtcg 3361 atttttcaac acttgttgac ttcaaaatgt tttacatcac cttttctata cggctgcata 3421 attcgaattt tcaatttgat ttgaatattc cggaaattcg ttgcttttta ctgcaaaaaa 3481 atagccgcgg cgcgtactcc tcagcgctcc tcgactcatt ctctctgaat ctcttgtata 3541 tcttctcact tttcttttct ttcccttttc gcccattttt gtgttatttt gtcgtttgga 3601 aaaggaaaag gagagaagag aggataggat gaacactaaa tactcttcgt tttccatgtt 3661 ttgttcactt ttagaaacaa agattccgcc gttttgtttg tttggtttca acaatgccat 3721 tttgagcaag tttgcgcttt tcgttccgct tcatcctgca aatttgaaaa gtttcacttt 3781 tcaagaacac ctgtgaaatc ccgtcgtggc ttccggagat tacacttttt gtctcttgtt 3841 ctcaaaacta actctcaaac atctttacgc caacgccagt tgctctcagt ttgaatcgaa 3901 aaaaacaaga ttcgtagaaa tttttccagt ttatttattg attttcaaaa ttcgaaatta 3961 gcctttattc caaatgaatt ttgctcgaat ttcatgatca acgctataaa ttcgaaacaa 4021 aaatcgtttt ttagagcgcg cgaaatttca taaggaattt acaattgctt ttcgaaaaaa 4081 ataactttcg aaacaataca aaaaactaaa aatatcaata ttttgttgga tgtcttcgtc 4141 tatatgttct tcccacagcc cttgtgttta tgtgtctcta gtacaaccgg cgacaacaca 4201 tttttttttg tctccagcag tgtcatacac aacccttcca ttttcttctc aaaatttgtc 4261 ataatatttt aaaatttcag ATGTCTTCCC ATCACAACGA CGTCATCGAA AAGAATTCAA 4321 CTTTGTATAC AAAAGTTGgG GAGGATCAGG AGGATCAGGA GGATCAGGAG GATCAGGAGG 4381 ATCAGGAGGA TCAGTGAGCA AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT 4441 CGAGCTGGAC GGCGACGTAA ACGGCCACAA GTTCAGCGTG TCCGGCGAGG GCGAGGGCGA 4501 TGCCACCTAC GGCAAGCTGA CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC 4561 CTGGCCCACC CTCGTGACCA CCCTGACCTA CGGCGTGCAG TGCTTCAGCC GCTACCCCGA 4621 CCACATGAAG CAGCACGACT TCTTCAAGTC CGCCATGCCC GAAGGCTACG TCCAGGAGCG 4681 CACCATCTTC TTCAAGGACG ACGGCAACTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG 4741 CGACACCCTG GTGAACCGCA TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT 4801 CCTGGGGCAC AAGCTGGAGT ACAACTACAA CAGCCACAAC GTCTATATCA TGGCCGACAA 4861 GCAGAAGAAC GGCATCAAGG TGAACTTCAA GATCCGCCAC AACATCGAGG ACGGCAGCGT 4921 GCAGCTCGCC GACCACTACC AGCAGAACAC CCCCATCGGC GACGGCCCCG TGCTGCTGCC 4981 CGACAACCAC TACCTGAGCA CCCAGTCCGC CCTGAGCAAA GACCCCAACG AGAAGCGCGA 5041 TCACATGGTC CTGCTGGAGT TCGTGACCGC CGCCGGGATC ACTCTCGGCA TGGACGAGCT 5101 GTACAAGTAA aACCCAGCTT TCTTGTACAA AGTGGTCGGC CGCTGTCATC AGATCGCCAT 5161 CTCGCGCCCG TGCCTCTGAC TTCTAAGTCC AATTACTCTT CAACATCCCT ACATGCTCTT 5221 TCTCCCTGTG CTCCCACCCC CTATTTTTGT TATTATCAAA AAACTTCTCT TAATTTCTTT 5281 GTTTTTTAGC TTCTTTTAAG TCACCTCTAA CAATGAAATT GTGTAGATTC AAAAATAGAA 5341 TTAATTCGTA ATAAAAAGTC GAAAAAAATT GTGCTCCCTC CCCCCATTAA TAATAATTCT 5401 ATCCCAAAAT CTACACAATG TTCTGTGTAC ACTTCTTATG TTTTTTACTT CTGATAAATT 5461 TTTTTTGAAA CATCATAGAA AAAACCGCAC ACAAAATACC TTATCATATG TTACGTTTCA 5521 GTTTATGACC GCAATTTTTA TTTCTTCGCA CGTCTGGGCC TCTCATGACG TCAAATCATG 5581 CTCATCGTGA AAAAGTTTTG GAGTATTTTT GGAATTTTTC AATCAAGTGA AAGTTTATGA 5641 AATTAATTTT CCTGCTTTTG CTTTTTGGGG GTTTCCCCTA TTGTTTGTCA AGAGTTTCGA 5701 GGACGGCGTT TTTCTTGCTA AAATCACAAG TATTGATGAG CACGATGCAA GAAAGATCGG 5761 AAGAAGGTTT GGGTTTGAGG CTCAGTGGAA GGTGAGTAGA AGTTGATAAT TTGAAAGTGG 5821 AGTAGTGTCT ATGGGGTTTT TGCCTTAAAT GACAGAATAC ATTCCCAATA TACCAAACAT 5881 AACTGTTTCC TACTAGTCGG CCGTACGGGC CCTTTCGTCT CGCGCGTTTC GGTGATGACG 5941 GTGAAAACCT CTGACACATG CAGCTCCCGG AGACGGTCAC AGCTTGTCTG TAAGCGGATG 6001 CCGGGAGCAG ACAAGCCCGT CAGGGCGCGT CAGCGGGTGT TGGCGGGTGT CGGGGCTGGC 6061 TTAACTATGC GGCATCAGAG CAGATTGTAC TGAGAGTGCA CCATATGCGG TGTGAAATAC 6121 CGCACAGATG CGTAAGGAGA AAATACCGCA TCAGGCGGCC TTAAGGGCCT CGTGATACGC 6181 CTATTTTTAT AGGTTAATGT CATGATAATA ATGGTTTCTT AGACGTCAGG TGGCACTTTT 6241 CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT 6301 CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT ATTGAAAAAG GAAGAGTATG 6361 AGTATTCAAC ATTTCCGTGT CGCCCTTATT CCCTTTTTTG CGGCATTTTG CCTTCCTGTT 6421 TTTGCTCACC CAGAAACGCT GGTGAAAGTA AAAGATGCTG AAGATCAGTT GGGTGCACGA 6481 GTGGGTTACA TCGAACTGGA TCTCAACAGC GGTAAGATCC TTGAGAGTTT TCGCCCCGAA 6541 GAACGTTTTC CAATGATGAG CACTTTTAAA GTTCTGCTAT GTGGCGCGGT ATTATCCCGT 6601 ATTGACGCCG GGCAAGAGCA ACTCGGTCGC CGCATACACT ATTCTCAGAA TGACTTGGTT 6661 GAGTACTCAC CAGTCACAGA AAAGCATCTT ACGGATGGCA TGACAGTAAG AGAATTATGC 6721 AGTGCTGCCA TAACCATGAG TGATAACACT GCGGCCAACT TACTTCTGAC AACGATCGGA 6781 GGACCGAAGG AGCTAACCGC TTTTTTGCAC AACATGGGGG ATCATGTAAC TCGCCTTGAT 6841 CGTTGGGAAC CGGAGCTGAA TGAAGCCATA CCAAACGACG AGCGTGACAC CACGATGCCT 6901 GTAGCAATGG CAACAACGTT GCGCAAACTA TTAACTGGCG AACTACTTAC TCTAGCTTCC 6961 CGGCAACAAT TAATAGACTG GATGGAGGCG GATAAAGTTG CAGGACCACT TCTGCGCTCG 7021 GCCCTTCCGG CTGGCTGGTT TATTGCTGAT AAATCTGGAG CCGGTGAGCG TGGGTCTCGC 7081 GGTATCATTG CAGCACTGGG GCCAGATGGT AAGCCCTCCC GTATCGTAGT TATCTACACG 7141 ACGGGGAGTC AGGCAACTAT GGATGAACGA AATAGACAGA TCGCTGAGAT AGGTGCCTCA 7201 CTGATTAAGC ATTGGTAACT GTCAGACCAA GTTTACTCAT ATATACTTTA GATTGATTTA 7261 AAACTTCATT TTTAATTTAA AAGGATCTAG GTGAAGATCC TTTTTGATAA TCTCATGACC 7321 AAAATCCCTT AACGTGAGTT TTCGTTCCAC TGAGCGTCAG ACCCCGTAGA AAAGATCAAA 7381 GGATCTTCTT GAGATCCTTT TTTTCTGCGC GTAATCTGCT GCTTGCAAAC AAAAAAACCA 7441 CCGCTACCAG CGGTGGTTTG TTTGCCGGAT CAAGAGCTAC CAACTCTTTT TCCGAAGGTA 7501 ACTGGCTTCA GCAGAGCGCA GATACCAAAT ACTGTCCTTC TAGTGTAGCC GTAGTTAGGC 7561 CACCACTTCA AGAACTCTGT AGCACCGCCT ACATACCTCG CTCTGCTAAT CCTGTTACCA 7621 GTGGCTGCTG CCAGTGGCGA TAAGTCGTGT CTTACCGGGT TGGACTCAAG ACGATAGTTA 7681 CCGGATAAGG CGCAGCGGTC GGGCTGAACG GGGGGTTCGT GCACACAGCC CAGCTTGGAG 7741 CGAACGACCT ACACCGAACT GAGATACCTA CAGCGTGAGC ATTGAGAAAG CGCCACGCTT 7801 CCCGAAGGGA GAAAGGCGGA CAGGTATCCG GTAAGCGGCA GGGTCGGAAC AGGAGAGCGC 7861 ACGAGGGAGC TTCCAGGGGG AAACGCCTGG TATCTTTATA GTCCTGTCGG GTTTCGCCAC 7921 CTCTGACTTG AGCGTCGATT TTTGTGATGC TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC 7981 GCCAGCAACG CGGCCTTTTT ACGGTTCCTG GCCTTTTGCT GGCCTTTTGC TCACATGTTC 8041 TTTCCTGCGT TATCCCCTGA TTCTGTGGAT AACCGTATTA CCGCCTTTGA GTGAGCTGAT 8101 ACCGCTCGCC GCAGCCGAAC GACCGAGCGC AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG 8161 CGCCCAATAC GCAAACCGCC TCTCCCCGCG CGTTGGCCGA TTCATTAATG CAGCTGGCAC 8221 GACAGGTTTC CCGACTGGAA AGCGGGCAGT GAGCGCAACG CAATTAATGT GAGTTAGCTC 8281 ACTCATTAGG CACCCCAGGC TTTACACTTT ATGCTTCCGG CTCGTATGTT GTGTGGAATT 8341 GTGAGCGGAT AACAATTTCA CACAGGAAAC AGCT //