LOCUS peft-3p-RET-1E4E 8362 bp DNA circular 04-SEP-2013 FEATURES Location/Qualifiers source 1..8362 /dnas_title="peft-3p-RET-1E4E5(+1)E6-GGS6-mCherry" promoter 23..627 /note="derived_from_eft-3" misc_feature 594..595 /note="SL1 trans-splice acceptor; see cm01d1.5" misc_feature 594..595 /note="SL2 trans-splice acceptor; see yk1281a09.5" mRNA join(595..650,693..770,2435..2516,4281..5349) /dnas_title="putative_exons_RET-1E4/E5(+1) /E6-GGS6-mCherry" mRNA join(595..650,693..770,4281..5349) /dnas_title="putative_exons_E5/E6(-1)-GGS6-mCherry" intron 651..692 /note="synthetic_intron" misc_recomb 708..732 /note="attB1" misc_recomb 716..722 /note="attB1 break" CDS join(738..770,4281..5099) /dnas_title="RET-1E4/E6-GGS6-mCherry" /translation="MVFGSLGSDDMMSSHHNDVIEKNSTLYTKVGGSGGSGGSGGSGG SGGSVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEGTQTAKLKVTK GGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVVTVT QDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEIKQRLK LKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERAEGRHSTG GMDELYK" CDS join(738..770,2435..2516,4281..4414) /dnas_title="RET-1E4/E5(+1)/E6*" /translation="MVFGSLGSDDMKPGSQDDGFVFIERNEANEATFKEESEDVFPSQ RRHRKEFNFVYKSWRIRRIRRIRRIRRIRRISEQGRGG" gene 741..4317 /note="derived from ret-1" variation 2500..2500 /note="+1 insertion" misc_recomb 4318..4338 /note="attB5" /note="CAACTTTGTATACAAAAGTTG" misc_recomb 4325..4331 /note="attB5 break" CDS 4338..4391 /note="(GGS)X6" CDS 4392..5099 /note="mCherry" /translation="VSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMN FEDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGA LKGEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQY ERAEGRHSTGGMDELYK" misc_recomb 5100..5124 /note="attB2" /note="ACCCAGCTTTCTTGTACAAAGTGGT" misc_recomb 5110..5116 /note="attB2 break" polyA_signal 5126..5876 /note="unc-54 3'_cassette" polyA_signal 5338..5343 /note="polyA" promoter 6247..6345 /dnas_title="bla promoter" regulatory 6276..6311 /dnas_title="AmpR promoter" /regulatory_class="promoter" /note="includes -35, -10, and transcription start site" /note="includes -35, -10, and transcription start site" CDS 6346..7206 /dnas_title="Ampicillin (bla) resistance gene" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 7354..7975 /note="pUC origin" regulatory 8289..8319 /dnas_title="lac promoter" /regulatory_class="promoter" /note="lac promoter" regulatory 8325..8345 /dnas_title="lac operator" /regulatory_class="transcriptional_cis_regulatory_region" /note="Lac repressor binding site" /note="Lac repressor binding site" ORIGIN 1 ATGACCATGA TTACGCCAAG CTtgtcaact tccattggtt cttccattgt ttctgttaaa 61 ttaatgaatt tttcataaaa taaagacatt atacaatata aaaatgaaga atttattgaa 121 aataaactgc cagagagaaa aagtatgcaa cactcccgcc gagagtgttt gaaatggtgt 181 acggtacatt ttcgtgctag gagttagatg tgcaggcagc aacgagaggg ggagagattt 241 ttttgggcct tgtgaaatta acgtgagttt tctggtcatc tgactaatca tgttggtttt 301 ttgttggttt attttgtttt tatctttgtt tttatccaga ttaggaaatt taaattttat 361 gaatttataa tgaggtcaaa cattcagtcc cagcgttttt cctgttctca ctgtttagtc 421 gaatttttat tttaggcttt caacaaatgt tctaactgtc ttatttgtga cctcactttt 481 tatatttttt taatttttaa aaatattaga agtttctagg ataatttttt cgacttttat 541 tctctctacc gtccgcactc ttcttacttt taaattaaat tgtttttttt tcagttggga 601 aacactttgc tcactccgta gcagccaGGG ATTGGCCAAA GGACCCAAAG gtatgtttcg 661 aatgatacta acataacata gaacattttc agGAGGACCC TTGGCTCACA AGTTTGTACA 721 AAAAAGCAGG CTccaccatg GTATTTGGAA GCCTTGGATC TGACGACATG gttagtttat 781 ttttatttgc aaaattttgg ttacatttta taatttaaac gaggaatgtg gtgaatgaat 841 tgaggaatct ttatggcctg tctctctagg tttgagcttt aaattttctg aaattaataa 901 ttgttaaagt caaatttaag gttcaaaacg tttgtgaatg cgttgaatgc ttgttcagta 961 tatttctatc ctacttttaa attcaatctg aaatcagata ggtcttatta gataggctta 1021 ggctgccaaa aattgtgctc ctgactgagt ttgaactggg aaactgtttt caaaatattt 1081 taaatttatc actttcgagt ccccgtgcaa ttgctccaag ttcaaatttt catttatttt 1141 ttatgatttt agagaaatag agaagtagga aaagatttag gatgcttgtt aggtttgttt 1201 ttcaattttg aaactttttt catcaaactc gatcaaaagt ctaaaatttt aattaggaaa 1261 gggacaactt tgattgattc taaaaaattt gaacaatttc gagtcggttt cttttagttc 1321 aaagatattt tctacagttt ttatagaagt ttggtgaaac tgaaaatctg aaattttata 1381 tttacaatta gtaaaagtct atcaatgact cttacactaa ataaaccctt ccaaaaaaag 1441 tccatataac tgtctggaga tcaaatactt tttttctccc aacttttcac actctcatca 1501 aaaccatttc tcgtggaacc atcaattttt attggcttca ttctcgttcg attcttcctc 1561 ttttccatca ccaaatgcac atacaaactc gcggaaaaaa gaaactaaaa gccaaacaaa 1621 tcgttacttt tctatccttt tccgaggtgg aaaaaagggt gacgctccac cacaatttta 1681 taaaaggaag attggagatt ccgtgaacct gcccattttc gttccttttc tgttcctttt 1741 gggactagtc gtccatgtaa aattataaaa ttgcgctgcg ccgcatacac tcgcatactt 1801 ttgccccttc ttctctctaa ggctttttgg tggtaactgg atagtggaag attctcaaat 1861 ttcttcgtct ttttccatat tttactacta agatataagt gagccacgag ctgctatgat 1921 tgtcaaatca tgacaatgat tttcggtgct tcttctgtaa ttatttctga aaatttggat 1981 gacgaatcag agagacaggt cttggtttat tatcagtttg tcaaaaaata tattgtgttg 2041 aaaataatgt cgccccaata attttgcaag cctgccataa agttaatcgt gtcgattcta 2101 ttaggacttg tgtagtccag gaggagaagt gttcatttat aattttaaat gttttttttg 2161 tatttaaaaa caatgaataa tggattgatc attttgaatg ttgagttcag aagagtttgg 2221 cgaaaaactg aaaattattt taatacatta aaacttttcc tagtaaaata catcgtaaat 2281 tggcatgata aaccgtgtgg cttgcaaaat tcaaggagct ctagcaggac aaaatgggaa 2341 actgatatat attggtgttg caagtgacta tattttcaaa aaacttggat attgtaaaaa 2401 tgcatgaatt tgtgaggtga tgtgtctaat tcagAAGCCA GGATCTCAGG ACGACGGATT 2461 CGTATTCATC GAACGAAACG AGGCAAACGA AGCAACTTTt AAAGAAGAAT CAGAAGgttg 2521 tgaatgagtt actttttctg tttttttttt agttttgttc tatgtctgtc agctccttaa 2581 tttgttgtag aactgtcagt gtttgtcaag tacacccaac cgctcctttt tgctatatca 2641 catgacccca atggaacaac caacaaacga aaaatatatt ttgagaaaaa ccggaaaggt 2701 gatcgcaaaa aaatattttc tcattttatt ttggtttgat agtgcggtaa tcaaagcgag 2761 tgtgaccgaa gaaaatcggt tgattactca ttgctatcga acactttttt actttgcaat 2821 actttatgat gactcattgg gaggcgtttg ctagattttt taacaagaat ttgaattatt 2881 ttcttttaat ccaaaaaaat tttattttat tttttctatt tttgttgatt tttcgataat 2941 tgaattcaga aaaaccaaca aaacgtacga aaaagaaatg aacttcgatg ttttgcaaaa 3001 aatattaaag cgcgcggcga gacccatccg cagaatttaa ccacctccct tgaataccgt 3061 agcatttctc aagaaaacac attttatttt caaatttaat tttttctgaa ttttacaagt 3121 gaatcctcat tgcggcgcca ctattttatt tttaatattt cacaactcaa aatttaaaga 3181 aaaaagtatt gctcacaaat tctcagcaat atttgttaaa actgttcaaa cctgcaaact 3241 ttcgaaacac tctccaaaag tgtcagcaat taatgagtca attttccgcg cgcgccacga 3301 gaaaattgct ttggaactgc ccaaagaccg cccgttctct tgttttccgg tcactcgtcg 3361 atttttcaac acttgttgac ttcaaaatgt tttacatcac cttttctata cggctgcata 3421 attcgaattt tcaatttgat ttgaatattc cggaaattcg ttgcttttta ctgcaaaaaa 3481 atagccgcgg cgcgtactcc tcagcgctcc tcgactcatt ctctctgaat ctcttgtata 3541 tcttctcact tttcttttct ttcccttttc gcccattttt gtgttatttt gtcgtttgga 3601 aaaggaaaag gagagaagag aggataggat gaacactaaa tactcttcgt tttccatgtt 3661 ttgttcactt ttagaaacaa agattccgcc gttttgtttg tttggtttca acaatgccat 3721 tttgagcaag tttgcgcttt tcgttccgct tcatcctgca aatttgaaaa gtttcacttt 3781 tcaagaacac ctgtgaaatc ccgtcgtggc ttccggagat tacacttttt gtctcttgtt 3841 ctcaaaacta actctcaaac atctttacgc caacgccagt tgctctcagt ttgaatcgaa 3901 aaaaacaaga ttcgtagaaa tttttccagt ttatttattg attttcaaaa ttcgaaatta 3961 gcctttattc caaatgaatt ttgctcgaat ttcatgatca acgctataaa ttcgaaacaa 4021 aaatcgtttt ttagagcgcg cgaaatttca taaggaattt acaattgctt ttcgaaaaaa 4081 ataactttcg aaacaataca aaaaactaaa aatatcaata ttttgttgga tgtcttcgtc 4141 tatatgttct tcccacagcc cttgtgttta tgtgtctcta gtacaaccgg cgacaacaca 4201 tttttttttg tctccagcag tgtcatacac aacccttcca ttttcttctc aaaatttgtc 4261 ataatatttt aaaatttcag ATGTCTTCCC ATCACAACGA CGTCATCGAA AAGAATTCAA 4321 CTTTGTATAC AAAAGTTGga ggatcaggag gatcaggagg atcaggagga tcaggaggat 4381 caggaggatc aGTGAGCAAG GGCGAGGAGG ATAACATGGC CATCATCAAG GAGTTCATGC 4441 GCTTCAAGGT GCACATGGAG GGCTCCGTGA ACGGCCACGA GTTCGAGATC GAGGGCGAGG 4501 GCGAGGGCCG CCCCTACGAG GGCACCCAGA CCGCCAAGCT GAAGGTGACC AAGGGTGGCC 4561 CCCTGCCCTT CGCCTGGGAC ATCCTGTCCC CTCAGTTCAT GTACGGCTCC AAGGCCTACG 4621 TGAAGCACCC CGCCGACATC CCCGACTACT TGAAGCTGTC CTTCCCCGAG GGCTTCAAGT 4681 GGGAGCGCGT GATGAACTTC GAGGACGGCG GCGTGGTGAC CGTGACCCAG GACTCCTCCC 4741 TGCAGGACGG CGAGTTCATC TACAAGGTGA AGCTGCGCGG CACCAACTTC CCCTCCGACG 4801 GCCCCGTAAT GCAGAAGAAG ACCATGGGCT GGGAGGCCTC CTCCGAGCGG ATGTACCCCG 4861 AGGACGGCGC CCTGAAGGGC GAGATCAAGC AGAGGCTGAA GCTGAAGGAC GGCGGCCACT 4921 ACGACGCTGA GGTCAAGACC ACCTACAAGG CCAAGAAGCC CGTGCAGCTG CCCGGCGCCT 4981 ACAACGTCAA CATCAAGTTG GACATCACCT CCCACAACGA GGACTACACC ATCGTGGAAC 5041 AGTACGAACG CGCCGAGGGC CGCCACTCCA CCGGCGGCAT GGACGAGCTG TACAAGTAGA 5101 CCCAGCTTTC TTGTACAAAG TGGTCGGCCG CTGTCATCAG ATCGCCATCT CGCGCCCGTG 5161 CCTCTGACTT CTAAGTCCAA TTACTCTTCA ACATCCCTAC ATGCTCTTTC TCCCTGTGCT 5221 CCCACCCCCT ATTTTTGTTA TTATCAAAAA ACTTCTCTTA ATTTCTTTGT TTTTTAGCTT 5281 CTTTTAAGTC ACCTCTAACA ATGAAATTGT GTAGATTCAA AAATAGAATT AATTCGTAAT 5341 AAAAAGTCGA AAAAAATTGT GCTCCCTCCC CCCATTAATA ATAATTCTAT CCCAAAATCT 5401 ACACAATGTT CTGTGTACAC TTCTTATGTT TTTTACTTCT GATAAATTTT TTTTGAAACA 5461 TCATAGAAAA AACCGCACAC AAAATACCTT ATCATATGTT ACGTTTCAGT TTATGACCGC 5521 AATTTTTATT TCTTCGCACG TCTGGGCCTC TCATGACGTC AAATCATGCT CATCGTGAAA 5581 AAGTTTTGGA GTATTTTTGG AATTTTTCAA TCAAGTGAAA GTTTATGAAA TTAATTTTCC 5641 TGCTTTTGCT TTTTGGGGGT TTCCCCTATT GTTTGTCAAG AGTTTCGAGG ACGGCGTTTT 5701 TCTTGCTAAA ATCACAAGTA TTGATGAGCA CGATGCAAGA AAGATCGGAA GAAGGTTTGG 5761 GTTTGAGGCT CAGTGGAAGG TGAGTAGAAG TTGATAATTT GAAAGTGGAG TAGTGTCTAT 5821 GGGGTTTTTG CCTTAAATGA CAGAATACAT TCCCAATATA CCAAACATAA CTGTTTCCTA 5881 CTAGTCGGCC GTACGGGCCC TTTCGTCTCG CGCGTTTCGG TGATGACGGT GAAAACCTCT 5941 GACACATGCA GCTCCCGGAG ACGGTCACAG CTTGTCTGTA AGCGGATGCC GGGAGCAGAC 6001 AAGCCCGTCA GGGCGCGTCA GCGGGTGTTG GCGGGTGTCG GGGCTGGCTT AACTATGCGG 6061 CATCAGAGCA GATTGTACTG AGAGTGCACC ATATGCGGTG TGAAATACCG CACAGATGCG 6121 TAAGGAGAAA ATACCGCATC AGGCGGCCTT AAGGGCCTCG TGATACGCCT ATTTTTATAG 6181 GTTAATGTCA TGATAATAAT GGTTTCTTAG ACGTCAGGTG GCACTTTTCG GGGAAATGTG 6241 CGCGGAACCC CTATTTGTTT ATTTTTCTAA ATACATTCAA ATATGTATCC GCTCATGAGA 6301 CAATAACCCT GATAAATGCT TCAATAATAT TGAAAAAGGA AGAGTATGAG TATTCAACAT 6361 TTCCGTGTCG CCCTTATTCC CTTTTTTGCG GCATTTTGCC TTCCTGTTTT TGCTCACCCA 6421 GAAACGCTGG TGAAAGTAAA AGATGCTGAA GATCAGTTGG GTGCACGAGT GGGTTACATC 6481 GAACTGGATC TCAACAGCGG TAAGATCCTT GAGAGTTTTC GCCCCGAAGA ACGTTTTCCA 6541 ATGATGAGCA CTTTTAAAGT TCTGCTATGT GGCGCGGTAT TATCCCGTAT TGACGCCGGG 6601 CAAGAGCAAC TCGGTCGCCG CATACACTAT TCTCAGAATG ACTTGGTTGA GTACTCACCA 6661 GTCACAGAAA AGCATCTTAC GGATGGCATG ACAGTAAGAG AATTATGCAG TGCTGCCATA 6721 ACCATGAGTG ATAACACTGC GGCCAACTTA CTTCTGACAA CGATCGGAGG ACCGAAGGAG 6781 CTAACCGCTT TTTTGCACAA CATGGGGGAT CATGTAACTC GCCTTGATCG TTGGGAACCG 6841 GAGCTGAATG AAGCCATACC AAACGACGAG CGTGACACCA CGATGCCTGT AGCAATGGCA 6901 ACAACGTTGC GCAAACTATT AACTGGCGAA CTACTTACTC TAGCTTCCCG GCAACAATTA 6961 ATAGACTGGA TGGAGGCGGA TAAAGTTGCA GGACCACTTC TGCGCTCGGC CCTTCCGGCT 7021 GGCTGGTTTA TTGCTGATAA ATCTGGAGCC GGTGAGCGTG GGTCTCGCGG TATCATTGCA 7081 GCACTGGGGC CAGATGGTAA GCCCTCCCGT ATCGTAGTTA TCTACACGAC GGGGAGTCAG 7141 GCAACTATGG ATGAACGAAA TAGACAGATC GCTGAGATAG GTGCCTCACT GATTAAGCAT 7201 TGGTAACTGT CAGACCAAGT TTACTCATAT ATACTTTAGA TTGATTTAAA ACTTCATTTT 7261 TAATTTAAAA GGATCTAGGT GAAGATCCTT TTTGATAATC TCATGACCAA AATCCCTTAA 7321 CGTGAGTTTT CGTTCCACTG AGCGTCAGAC CCCGTAGAAA AGATCAAAGG ATCTTCTTGA 7381 GATCCTTTTT TTCTGCGCGT AATCTGCTGC TTGCAAACAA AAAAACCACC GCTACCAGCG 7441 GTGGTTTGTT TGCCGGATCA AGAGCTACCA ACTCTTTTTC CGAAGGTAAC TGGCTTCAGC 7501 AGAGCGCAGA TACCAAATAC TGTCCTTCTA GTGTAGCCGT AGTTAGGCCA CCACTTCAAG 7561 AACTCTGTAG CACCGCCTAC ATACCTCGCT CTGCTAATCC TGTTACCAGT GGCTGCTGCC 7621 AGTGGCGATA AGTCGTGTCT TACCGGGTTG GACTCAAGAC GATAGTTACC GGATAAGGCG 7681 CAGCGGTCGG GCTGAACGGG GGGTTCGTGC ACACAGCCCA GCTTGGAGCG AACGACCTAC 7741 ACCGAACTGA GATACCTACA GCGTGAGCAT TGAGAAAGCG CCACGCTTCC CGAAGGGAGA 7801 AAGGCGGACA GGTATCCGGT AAGCGGCAGG GTCGGAACAG GAGAGCGCAC GAGGGAGCTT 7861 CCAGGGGGAA ACGCCTGGTA TCTTTATAGT CCTGTCGGGT TTCGCCACCT CTGACTTGAG 7921 CGTCGATTTT TGTGATGCTC GTCAGGGGGG CGGAGCCTAT GGAAAAACGC CAGCAACGCG 7981 GCCTTTTTAC GGTTCCTGGC CTTTTGCTGG CCTTTTGCTC ACATGTTCTT TCCTGCGTTA 8041 TCCCCTGATT CTGTGGATAA CCGTATTACC GCCTTTGAGT GAGCTGATAC CGCTCGCCGC 8101 AGCCGAACGA CCGAGCGCAG CGAGTCAGTG AGCGAGGAAG CGGAAGAGCG CCCAATACGC 8161 AAACCGCCTC TCCCCGCGCG TTGGCCGATT CATTAATGCA GCTGGCACGA CAGGTTTCCC 8221 GACTGGAAAG CGGGCAGTGA GCGCAACGCA ATTAATGTGA GTTAGCTCAC TCATTAGGCA 8281 CCCCAGGCTT TACACTTTAT GCTTCCGGCT CGTATGTTGT GTGGAATTGT GAGCGGATAA 8341 CAATTTCACA CAGGAAACAG CT //